cells-to-ct 1-step taqman kit (Thermo Fisher)

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Thermo Fisher cells-to-ct 1-step taqman kit
Cells To Ct 1 Step Taqman Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cells+to+ct+kit/powerup+sybr+green+master+mix/pmc10990115__pnas__2313672121__sapp-37-21-25
Average 90 stars, based on 1 article reviews

cells-to-ct 1-step taqman kit - by Bioz Stars, 2026-09

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Article Title: Global kinome silencing combined with 3D invasion screening of the tumor microenvironment identifies fibroblast-expressed PIK3Cδ involvement in triple-negative breast cancer progression
Article Snippet: Part of the transfected cells were used to determine the efficacy of silencing using Cells To CT kit (Thermofisher), following manufacturer’s instruction for Real-time PCR assay (qRT-PCR), using specific primers for each silenced kinase (QuantiTect, #249900, Qiagen).

Article Title: Altered acetyl-CoA metabolism presents a new potential immunotherapy target in the obese lung microenvironment.
Article Snippet: Tregs recovered from lung tissues were directly lysed and converted to cDNA using the “Cells to CT” kit (Thermo) following the manufacturer’s protocol.

Article Title: NBS1 I171V variant underlies individual differences in chromosomal radiosensitivity within human populations
Article Snippet: Briefly, HCT116 genome editing cells in 96-well plates were cultured in DMEM supplemented with 10% FBS for 24 h. The cells were washed with ice-cold PBS, lysed, and then, cDNAs were synthesized with reverse transcriptase from their extracted total RNA using Cells to CT Kit (ThermoFisher), in accordance with the manufacturer's protocol.

Article Title: Circulating heparan sulfate fragments attenuate histone-induced lung injury independently of histone binding
Article Snippet: The reverse transcription reactions were carried out using a Cells-to-CTTM kit (Invitrogen) with 7300 Real Time PCR System (AB Applied Biosystems).

Article Title: T-type Calcium Channels Determine the Vulnerability of Dopaminergic Neurons to Mitochondrial Stress in Familial Parkinson Disease
Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to measure the level of gene expression 72 h after the transfection using the Cells to CT Kit (Thermo Fisher Scientific).

Isolation:

Article Title: Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage
Article Snippet: .. When cells reached ∼80% confluency (3-4 days post-seeding), RNA isolation and cDNA generation was performed using the Cells-to-CT kit (Invitrogen, #4402954), per the manufacturer’s instructions. .. Real-time quantitative PCR was performed using the POWER SYBR Green PCR Master Mix (Applied Biosystems, #4367659) to measure expression levels of GUSB, TERT , GABPB1L , total GABPB1 , GABPB1S , GABPB2 , RPA1, and MGMT .

Gene Expression:

Article Title: Dependence on the Pyrimidine Biosynthetic Enzyme DHODH Is a Synthetic Lethal Vulnerability in Mutant KRAS-Driven Cancers.
Article Snippet: .. Downregulation of KRAS at the mRNA level was determined using a KRAS Taqman Gene Expression assay - Hs00270666_m1 (Thermofisher) and Cells-To-Ct kit (Ambion/ABI, #4399002). .. RPL37A - Hs01102345_m1 (ThermoFisher) was used as a housekeeping gene for normalization.

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